NanoOne Bio
Customized beyond precise bioprinting
NanoOne systems offer both a standard configuration and the flexibility to customize with additional modules to meet specific application demands. Easily explore upgrade options to ensure your NanoOne Bio package meets your requirements. These upgrades can be retrofitted at any time, ensuring your system evolves alongside your innovative manufacturing approaches. NanoOne Bio’s capabilities extend beyond bioprinting, making it versatile for various applications.
See THE Nano One BIO in action
Design with a focus on biological applications
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Cell-containing samples for three-dimensional in-vitro tests
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Filter elements with defined pore sizes and gyroid structures
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Barrier models with defined semi-permeability within microfluidic chips
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Scaffold structures positioned in each individual well of a cell culture plate
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Cell models mimicking natural tissue topography and microenvironment
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Medical devices such as implants or microneedle arrays
Specifications of the system
The only true incubation system for 2PP-3D printing systems
The UpNano BioUnit is the centerpiece of the NanoOne’s bio variant and an upgrade opportunity for all printing systems. It’s the only true incubation system for high-resolution 3D printing, providing a native, stress-free environment for the living cells during the printing process. The stage-top incubation system offers a plethora of features, allowing adjustments to match the preferred environmental conditions of the respective cell type and ensures these conditions during printing.
High-resolution bioprinting with living cells
Our comprehensive incubation system comprises of two controller units, a heating panel, and a CO2 gas incubation system. The gas incubation system provides a regulated gas atmosphere along with precise humidity levels within a continuous gas stream circulated through the incubation chamber. Meanwhile the heating panel maintains the ambient temperature required by the specific cell type, ensuring a native environment during the printing process and thus preserving cell viability.
Experience the future of bioprinting with NanoOne
Direct printing of living cells for cutting-edge research, unrestricted substrate options, and precise control of environmental conditions for optimal cell viability.
High-resolution bioprinting with the NanoOne printing system enables direct printing of living cells. Cells embedded into the printing resin can be used for three-dimensional in-vitro cell tests and high-resolution 3D bioprinting, which have become increasingly important in cell culture, tissue regeneration and pharmaceutical research. There is hardly any restriction in the choice of substrate – cell culture plates, petri dishes or microfluidic chips with glass bottoms can be inserted into the system. In combination with the BioUnit native temperature, humidity, and CO2 conditions are maintained during the printing process, ensuring cell viability.
Revolutionize the manufacturing of 3D scaffold structures
Biocompatible resins, perfect for mimicking cellular microenvironments and setting new standards in preclinical research and therapeutic strategies.
Sterile-manufactured scaffolds with UpNano’s biocompatible resins enable the fabrication of structures and surface textures mimicking the microenvironment of cells. These three-dimensional culture approaches, along with the results obtained from them, are becoming increasingly important in preclinical research and will be groundbreaking for future therapeutic strategies. In addition to the BIO INX® biofunctional material portfolio, five UpNano resins have been certified as non-cytotoxic and highly biocompatible according to EN ISO 10992-5:2009. Furthermore, the UpOpto and UpFlow resins exhibit low fluorescence, making them advantageous for cell biological applications and microscopy.
Rethinking production of microfluidic and microphysiological systems
UpNano’s dip-in free proprietary process unlocks new possibilities in 3D printing directly within a microfluidic chip or cell culture well.
This offers high-precision manufacturing for microfluidic and microphysiological systems. With this innovative approach, producing geometries within substrates, as well as entire chip designs with complex internal channel structures as small as 10 µm, has never been easier and safer for cells. This allows for contamination-free printing of elements such as separators, channels, membranes, or scaffolds, with the material prepared and post-processed under the cell culture hood.
Examples of compatible cell-culture substrates
Biocompatible 3D printing resins according to EN ISO 10993-5
In addition to a cell-compatible process, the choice of printing resin is crucial. With our collaboration with the Belgian company BIO INX®, we have developed a biofunctional material portfolio that is not only cell-compatible but also optimized for the NanoOne platform. Moreover, UpNano offers a portfolio of five biocompatible resins certified as non-cytotoxic according to EN ISO 10993-5:2009. Each of these resins, in addition to being biocompatible, has unique properties that highlight the wide range of possible applications. Choose UpNano for both cell compatibility and versatility in resin selection.
Questions & Answers
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The NanoOne Bio is tailored for biomedical applications by enabling high-resolution printing of biocompatible, cell-friendly resins. It allows for the creation of detailed cellular microenvironments and tissue scaffolds that support cell growth, making it ideal for bioprinting and tissue engineering research.
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Yes, the NanoOne Bio can print directly in the presence of living cells. UpNano provides a gelatin-based bioink, Hydrobio INX U200, that was co-developed with BIO INX specifically for the NanoOne. It offers the highest throughput, resolution and biocompatibility of any commercially available 2PP bioink. Users can also use their own hydrogels (e.g. methacrylated gelatin or hyaluronic acid) for cell embedding. This capability allows for the creation of cell models that mimic natural tissue environments, advancing research in tissue engineering and regenerative medicine.
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Creating scaffolds, tissue models and organ-on-chip systems with applications such as tissue regeneration or drug and toxicity testing. The high-resolution and versatility of the NanoOne Bio allows researchers to precisely modify the mechanical and chemical properties of the cells’ matrix. Hydrogels can be further modified after crosslinking with photoablation, photocleaving or photopatterning. Tissue-relevant architectures, such as perfusable microvessels, pulmonary alveoli or the intestinal crypts can be fabricated even directly inside of microfluidic chips.
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The NanoOne Bio achieves ultra-high precision for 3D bioprinting, with a resolution below 150 nanometers. This capability enables the creation of intricate structures at both the micro and nanoscale, essential for applications in tissue engineering and regenerative medicine.
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The NanoOne Bio system is highly customizable, designed to adapt to evolving needs. It offers a standard configuration that can be enhanced with additional modules, such as fiber holders, wafer chucks or heatable material vats. These upgrades can be retrofitted at any time, ensuring the system evolves alongside your innovative manufacturing approaches.
Additionally, the NanoOne Bio features an integrated Bio Unit which is the only true incubation system for high-resolution 2PP 3D printing. This system allows precise control over environmental conditions, including temperature, humidity, and CO₂ concentration, to match the specific requirements of different cell types during the printing process.
This combination of modular hardware and environmental control makes the NanoOne Bio a versatile platform for a wide range of biomedical applications, from tissue engineering to pharmaceutical research. -
Scaffolds, tissue constructs and organ-on-a-chip devices are common applications for which the NanoOne Bio is used. Tissue-specific structures, such as microvessels, pulmonary alveoli, hepatic lobules, nephrons, barriers or intestinal crypts can also be fabricated with the NanoOne Bio.
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The incubation system of the NanoOne Bio maintains a controlled environment with precise temperature, humidity and CO₂ levels during printing. This ensures optimal conditions for live cell bioprinting and preserves cell viability within delicate bioresins throughout the fabrication process.
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The NanoOne Bio is equipped with a 1000 mW (1 W) femtosecond fiber laser operating at a wavelength of 780 nm.
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The NanoOne Bio stands out by combining ultra-high resolution two-photon polymerization with an integrated incubation system that maintains live cell viability during printing. Unlike typical 3D printers, it enables direct printing of cell-laden, biocompatible structures at the micro- and nanoscale, ideal for advanced tissue engineering and biomedical research. It also pairs with custom features in our Think3D software including the Well-Plate Wizard which allows for printing of unique structures in multiple well-plates in a single print process.
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All photosensitive (bio)inks can be processed with the NanoOne Bio. For photoablation and photopatterning even non-photosensitive hydrogels, such as Matrigel or collagen, can be used. UpNano, together with their partner BIO INX, offers a range of resins that have been specifically developed for biological applications. Hydrobio INX U200 is a gelatin-based bioink that enables the embedding of live cells, while providing a high-resolution and high-throughput. Moreover, Hydrotech INX U200 is a bioinert technical hydrogel and Degrad INX U200 is a degradable polyester-based material. Many of the standard UpNano resins have also been certified as biocompatible according to ISO 10993-5. These materials enable printing of complex, high-resolution structures suitable for tissue engineering and organ-on-chip models.
