Experience the groundbreaking capabilities of the NanoOne bio package, the only 2PP printer with an integrated incubation system. The NanoOne’s bio variant combines the potential for biocompatible applications with the characteristic high precision and versatility of the NanoOne 1000, achieving unparalleled precision in 3D printing of structures in the presence of living cells. From creating cell models that mimic natural tissue topography to the sterile fabrication of cell scaffolds, NanoOne bio opens doors to a new era in biomedical research. Join us in shaping the future of bioprinting.
Customized beyond precise bioprinting
NanoOne systems offer both a standard configuration and the flexibility to customize with additional modules to meet specific application demands. Easily explore upgrade options to ensure your NanoOne bio package meets your requirements. These upgrades can be retrofitted at any time, ensuring your system evolves alongside your innovative manufacturing approaches. NanoOne Bio’s capabilities extend beyond bioprinting, making it versatile for various applications.
Design with a focus on biological applications
Specifications of the system
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System type
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Desktop multiphoton laser lithography system
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Printing process
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Layer-by-layer 2-photon polymerization
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Light source
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Femtosecond fiber laser
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Laser wavelength
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780 nm
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Output power of femtosecond laser
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1000 mW*
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Scanner
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Galvanometer scanner
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Stage
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Long-range piezo stage
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Maximum travel range
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Up to 120 x 100 x 49 mm
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Stage address grid
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10 nm
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System dimensions
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Benchtop system 94.0 x 71.0 x 65.0 cm³
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Total weight
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156 kg
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*Power in the sample depends on the selected objective and is lower than the output power of the laser. For further information, ask the UpNano sales team.
Overview of NanoOne models
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Output power of femtosecond laser
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250 mW*
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1000 mW*
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1000 mW*
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400 mW*
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Laser wavelength
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780 nm
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780 nm
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780 nm
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515 nm
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Pulse length
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90 fs
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90 fs
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90 fs
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90 fs
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Standard objectives
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40x NA 1.4
20x NA 0.7 10x NA 0.4 |
40x NA 1.4
20x NA 0.7 10x NA 0.4 5x NA 0.25 |
40x NA 1.4
20x NA 0.7 10x NA 0.4 10x NA 0.3 5x NA 0.25 |
40x NA 1.4
20x NA 0.7 10x NA 0.4 |
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System dimensions
Total weight |
58.5 x 71.0 x 65.0 cm³
124 kg |
58.5 x 71.0 x 65.0 cm³
124 kg |
94.0 x 71.0 x 65.0 cm³
156 kg |
58.5 x 71.0 x 65.0 cm³
124 kg |
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*Power in the sample depends on the selected objective and is lower than the output power of the laser. For further information, ask the UpNano sales team.
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System type
|
Desktop multiphoton laser lithography system
|
|---|---|
|
Printing process
|
Layer-by-layer 2-photon polymerization
|
|
Light source
|
Femtosecond fiber laser
|
|
Scanner
|
Galvanometer scanner
|
|
Stage
|
Long-range piezo stage
|
|
Maximum travel range
|
Up to 120 x 100 x 49 mm
|
|
Stage address grid
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10 nm
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The only true incubation system for 2PP-3D printing systems
The UpNano BioUnit is the centerpiece of the NanoOne’s bio variant and an upgrade opportunity for all printing systems. It’s the only true incubation system for high-resolution 3D printing, providing a native, stress-free environment for the living cells during the printing process. The stage-top incubation system offers a plethora of features, allowing adjustments to match the preferred environmental conditions of the respective cell type and ensures these conditions during printing.
High-resolution bioprinting with living cells
Our comprehensive incubation system comprises of two controller units, a heating panel, and a CO2 gas incubation system. The gas incubation system provides a regulated gas atmosphere along with precise humidity levels within a continuous gas stream circulated through the incubation chamber. Meanwhile the heating panel maintains the ambient temperature required by the specific cell type, ensuring a native environment during the printing process and thus preserving cell viability.
Experience the future of bioprinting with NanoOne
Direct printing of living cells for cutting-edge research, unrestricted substrate options, and precise control of environmental conditions for optimal cell viability.
Revolutionize the manufacturing of 3D scaffold structures
Biocompatible resins, perfect for mimicking cellular microenvironments and setting new standards in preclinical research and therapeutic strategies.
Rethinking production of microfluidic and microphysiological systems
UpNano’s dip-in free proprietary process unlocks new possibilities in 3D printing directly within a microfluidic chip or cell culture well.
Examples of compatible cell-culture substrates
Biocompatible 3D printing resins according to EN ISO 10993-5
In addition to a cell-compatible process, the choice of printing resin is crucial. With our collaboration with the Belgian company BIO INX®, we have developed a biofunctional material portfolio that is not only cell-compatible but also optimized for the NanoOne platform. Moreover, UpNano offers a portfolio of five biocompatible resins certified as non-cytotoxic according to EN ISO 10993-5:2009. Each of these resins, in addition to being biocompatible, has unique properties that highlight the wide range of possible applications. Choose UpNano for both cell compatibility and versatility in resin selection.
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Frequently asked questions
The NanoOne bio is tailored for biomedical applications by enabling high-resolution printing of biocompatible, cell-friendly resins. It allows for the creation of detailed cellular microenvironments and tissue scaffolds that support cell growth, making it ideal for bioprinting and tissue engineering research.
Yes, the NanoOne bio can print directly in the presence of living cells. UpNano provides a gelatin-based bioink, Hydrobio INX U200, that was co-developed with BIO INX specifically for the NanoOne. It offers the highest throughput, resolution and biocompatibility of any commercially available 2PP bioink. Users can also use their own hydrogels (e.g. methacrylated gelatin or hyaluronic acid) for cell embedding. This capability allows for the creation of cell models that mimic natural tissue environments, advancing research in tissue engineering and regenerative medicine.
Creating scaffolds, tissue models and organ-on-chip systems with applications such as tissue regeneration or drug and toxicity testing. The high-resolution and versatility of the NanoOne Bio allows researchers to precisely modify the mechanical and chemical properties of the cells’ matrix. Hydrogels can be further modified after crosslinking with photoablation, photocleaving or photopatterning. Tissue-relevant architectures, such as perfusable microvessels, pulmonary alveoli or the intestinal crypts can be fabricated even directly inside of microfluidic chips.
The NanoOne bio achieves ultra-high precision for 3D bioprinting, with a resolution below 150 nanometers. This capability enables the creation of intricate structures at both the micro and nanoscale, essential for applications in tissue engineering and regenerative medicine.
The NanoOne bio system is highly customizable, designed to adapt to evolving needs. It offers a standard configuration that can be enhanced with additional modules, such as fiber holders, wafer chucks or heatable material vats. These upgrades can be retrofitted at any time, ensuring the system evolves alongside your innovative manufacturing approaches.
Additionally, the NanoOne bio features an integrated Bio Unit which is the only true incubation system for high-resolution 2PP 3D printing. This system allows precise control over environmental conditions, including temperature, humidity, and CO₂ concentration, to match the specific requirements of different cell types during the printing process.
This combination of modular hardware and environmental control makes the NanoOne bio a versatile platform for a wide range of biomedical applications, from tissue engineering to pharmaceutical research.
Scaffolds, tissue constructs and organ-on-a-chip devices are common applications for which the NanoOne bio is used. Tissue-specific structures, such as microvessels, pulmonary alveoli, hepatic lobules, nephrons, barriers or intestinal crypts can also be fabricated with the NanoOne bio.
The incubation system of the NanoOne bio maintains a controlled environment with precise temperature, humidity and CO₂ levels during printing. This ensures optimal conditions for live cell bioprinting and preserves cell viability within delicate bioresins throughout the fabrication process.
The NanoOne bio is equipped with a 1000 mW (1 W) femtosecond fiber laser operating at a wavelength of 780 nm.
The NanoOne bio stands out by combining ultra-high resolution two-photon polymerization with an integrated incubation system that maintains live cell viability during printing. Unlike typical 3D printers, it enables direct printing of cell-laden, biocompatible structures at the micro- and nanoscale, ideal for advanced tissue engineering and biomedical research. It also pairs with custom features in our Think3D software including the Well-Plate Wizard which allows for printing of unique structures in multiple well-plates in a single print process.
• All photosensitive (bio)inks can be processed with the NanoOne bio. For photoablation and photopatterning even non-photosensitive hydrogels, such as Matrigel or collagen, can be used. UpNano, together with their partner BIO INX, offers a range of resins that have been specifically developed for biological applications. Hydrobio INX U200 is a gelatin-based bioink that enables the embedding of live cells, while providing a high-resolution and high-throughput. Moreover, Hydrotech INX U200 is a bioinert technical hydrogel and Degrad INX U200 is a degradable polyester-based material. Many of the standard UpNano resins have also been certified as biocompatible according to ISO 10993-5. These materials enable printing of complex, high-resolution structures suitable for tissue engineering and organ-on-chip models.
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